Figure 5.

Identification of optimal electroporation conditions: (a) On-chip lysis using a 1 V field for five different time intervals: (i) yield and purity results (black bars, relative yield; grey bars, relative fluorescence) and (ii) NABSA amplification data. Triangles, positive control; white circles, 1 s; crosses, 30 s; black circles, 60 s; plus symbols, 90 s; squares, 120 s. Increasing duration of electroporation led to increased yield of RNA extracted from the cell lysate. (b) On-chip lysis using a 30 V field for five different time intervals: (i) Yield and purity results (black bars, relative yield; grey bars, relative fluorescence) and (ii) NABSA amplification data. White triangles, positive control; diamonds, 1 s; squares, 30 s; crosses, 60 s; circles, 90 s; black triangles, 120 s. Electroporation that lasted longer than 60 s caused the yield of RNA extracted from the cell lysate to decrease. (c) On-chip lysis using a 60 V field for different time intervals: (i) Yield and purity results (black-filled bars, relative yield; grey-filled bars, relative fluorescence) and (ii) NABSA amplification data. Black circles, positive control; triangles, 1 s; white circles, 30 s; crosses, 60 s. Data show that electroporation of 60 V was more effective than the bench-top alternative but appeared to degrade the quality of extracted RNA from the lysate.