Table 1.
Comparison of stepwise purity and yield of AGP purification methods.
| Total protein (mg) | AGP (mg) | AGP/total protein | Fold enrichment versus starting material | |
|---|---|---|---|---|
| Method 3 | ||||
| Plasma | 1786 | 17.1 | 0.0096 | 1.0 |
| Affi-Gel Blue FT | 86.6 | 17.8 | 0.21 | 21 |
| CHT Eluate | 16.3 | 9.1 | 0.56 | 58 |
| HiTrap Blue FT | 8.7 | 9.3 | 1.06 | 110 |
| Method 4 | ||||
| Plasma | 1786 | 17.7 | 0.0099 | 1.0 |
| SSA Supernatant | 43.3 | 13.3 | 0.31 | 31 |
| CHT Eluate | 20.8 | 7.8 | 0.38 | 38 |
| HiTrap Blue FT | 9.19 | 8.1 | 0.89 | 89 |