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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2010 Dec 3;17(5):620–631. doi: 10.1016/j.bbmt.2010.11.029

Figure 4. Intensified PC regimen effectively constrains host immune recovery.

Figure 4

(a) Host B6 mice received either pentostatin (1 mg/kg on days 1, 4, 8 and 12) or fludarabine (100 mg/kg on days 1, 4, 8 and 12), with each drug administered in combination with cyclophosphamide (100 mg/kg/d; days 1 through 14). After treatment with these PC or FC regimens, spleen cells were harvested on days 0, 7 and 14 post-regimen and evaluated by flow cytometry for total number of CD4+ and CD8+ T cells and myeloid cells (Gr-1+). (b) Bone marrow cells were also harvested on days 0, 7 and 14 and analyzed for T cell composition and myeloid lineage. (c) On days 0, 7 and 14 post-regimen, spleen cells were stimulated with anti-CD3/CD28 beads and 24-hr supernatant was tested for IFN-γ and IL-2 content. All results are shown as mean ± SEM of n=5 per cohort for each time point.