Table 1.
Application | Primary Antibodies | Species | Dilution | Secondary Antibodies |
---|---|---|---|---|
Injury Index Mapping |
Anti-GFAP (Sigma) |
Mouse | 100 | Universal LSAB + System-HRP (DAKO) |
Anti-ATF3 (Sigma) |
Mouse | 50 | Universal LSAB + System-HRP (DAKO) |
|
| ||||
ATF3 Co- localization |
Anti-ATF3 (Sigma) |
Rabbit | 200 | Donkey anti rabbit + FITC (Jackson) |
Anti-GFAP (Sigma) |
Mouse | 500 | Donkey anti mouse + Red-X (Jackson) |
|
Anti-β-Tubulin III (Sigma) |
Mouse | 1000 | Donkey anti mouse + Red-X (Jackson) |
|
| ||||
NOSs Co- localization |
Anti-iNOS (BD bioscience) |
Mouse | 200 | Donkey anti mouse + Red-X (Jackson) |
Anti-ATF3 (Sigma) |
Rabbit | 200 | Donkey anti rabbit + FITC (Jackson) |
|
Anti-GFAP (Sigma) |
Rabbit | 200 | Donkey anti rabbit + FITC (Jackson) |
|
Anti-nNOS (Cell signaling) |
Rabbit | 100 | Donkey anti rabbit + FITC (Jackson) |
|
Anti-ATF3 (Sigma) |
Mouse | 200 | Donkey anti mouse + Red-X (Jackson) |
|
Anti-GFAP (Sigma) |
Mouse | 500 | Donkey anti mouse + Red-X (Jackson) |