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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Protein Expr Purif. 2010 Dec 28;77(1):80–85. doi: 10.1016/j.pep.2010.12.013

Fig. 2.

Fig. 2

Mapping the region in VP19C that causes the block in its expression. Plasmid pGEX4T3 that contains different gene truncations of VP19C were examined for expression in BL21 (RIPL) cells as described in legend to Fig.1. The numbers refer to N-terminal VP19C amino acids encoded by the plasmids or the prefix FL indicates the gene starts at the numbered amino acid and extends to the end of VP19C (residue 465). A: N-terminal polypeptides GST-VP19C-95 (10.1 kDa), 105 (11 kDa), 115 (12.2 kDa), 125 (13.3 kDa) and 135 (14.4 kDa) were expressed. B: N-terminal polypeptides GST-VP19C-140 (14.8 kDa) and 145 (15.9 kDa) expressed. C: C-terminal polypeptides GST-VP19C-150FL (34.7 kDa), 155FL (33.9 kDa) and 160FL (33.4 kDa) expressed. Gels shown in A and B were 15% acrylamide and in C was 12% acrylamide. Protein standards (kDa) are marked in lane M. Proteins derived from cells prior to induction are in lane UN.