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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Protein Expr Purif. 2010 Dec 28;77(1):80–85. doi: 10.1016/j.pep.2010.12.013

Fig. 4.

Fig. 4

Co-expression of the HSV-1 triplex proteins in the same cell. Rosetta (DE3) cells were transformed with pGEX-VP19CR146A (clones 3 and 5), pET-VP23 or with both VP19C and VP23 expressing plasmids. Exponential cultures were induced with IPTG and the cells harvested 2 h post-induction at 37°C. Protein lysates were analyzed by SDS-PAGE (15% acrylamide) and protein standards are in lane M). Proteins from uninduced cultures are shown in lane UN.

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