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. 2011 Mar 7;2011:549281. doi: 10.1155/2011/549281

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Copyright © 2011 Masaji Okada et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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ELISPOT assay for IFN-γ antigen-specific responses in the spleens of vaccinated mice following stimulation with rHSP65 protein. Spleen cell cultures were stimulated with rHSP65 protein for 20 h. The numbers of IFN-γ-secreting cells specific for rHSP65 protein per million cells were determined individually by ELISPOT assay. Results are expressed as the mean ± S.D. of 6 wells of 3 mice per group. The statistical significance of differences between individual groups in the number of IFN-γ-secreting cells was determined by Dunnett test. The statistical significance of differences (P < .01) of the G1 (naive) group compared to the G2 (BCG alone group), G3 (DNA/DNA/DNA), G4 (BCG/DNA/DNA), or G5 (DNA/DNA/BCG). The statistical significance of the G2 group difference (P < .01) compared to the G4 or the G5. The statistical significance of the G3 group differences (P < .01) compared to the G4. P < .01; the G4 group compared to the G5. The statistical significance of the G3 group differences (P < .05) compared to the G5.