HSP17 forms stable complexes with heat-denatured MDH. Increasing
amounts of MDH (0, 0.5, 1, 2, 4, 6, 8 μM) were heat-denatured (30 min
at 47°C) or kept at 25°C in buffer A containing 10 mM DTT in the
presence of HSP17 (16 μM) and analyzed by nondenaturing PAGE (6%).