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. 2001 Feb 27;98(6):3098–3103. doi: 10.1073/pnas.051619498

Figure 2.

Figure 2

Chaperone-assisted refolding of heat-denatured MDH. MDH (1.2 μM) was 99.8% heat-denatured during 30 min at 47°C, either in the absence (no) or in the presence of 12 μM HSP17. After heat shock (→) recovery of MDH, activity was followed at 25°C in the presence of ATP (1 mM), an active ATP-regeneration system, without additional chaperones (no), or with E. coli DnaK, DnaJ, GrpE, GroEL, GroES (4, 0.8, 0.4, 4, 5 μM, respectively). The final concentration of MDH and HSP17 was 1 μM and 10 μM, respectively.