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. 2001 Feb 27;98(6):3098–3103. doi: 10.1073/pnas.051619498

Figure 7.

Figure 7

Effect of lipids on the refolding of heat-denatured MDH by the multichaperone HSP17-KJELS system. MDH (1.2 μM) was denatured (30 min 47°C in buffer A containing 10 mM DTT) in the presence of HSP17 (7.2 μM). LUVs made of the indicated lipids were added to a final lipid concentration of 360 μM before the heat treatment. Recovery of MDH activity was followed at 25°C in the presence of ATP (1 mM), an active ATP-regeneration system supplemented with KJELS chaperones (derived from E. coli) (4, 0.8, 0.4, 4, and 6 μM, respectively). The final concentration of MDH, HSP17, and LUVs were 1 μM, 6 μM, and 300 μM, respectively.