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. Author manuscript; available in PMC: 2011 Mar 21.
Published in final edited form as: Adv Exp Med Biol. 2009;656:13–29. doi: 10.1007/978-1-4419-1145-2_2

Table 1a.

Evidence for nuclear APC

Observation Cell line(s) Antibodies Ref
APC in nuclear puncta by immunofluorescence (IF) 184A1, T47D Ab-2,-4,-6, APC64 1
microscopy (also cytoplasmic and at leading edge)
Cell fractionation/immunoblot: LS174T, HCT116 Ab-1, -2 1
APC in nuclear and nuclear matrix fractions
truncated APC not in nuclear fractions DLD-1 Ab-1 1
APC in nuclei of murine villus epithelial cells mouse tissue α-APC2 (middle) 5
IF with confocal microscopy
APC in nuclei of human colonic epithelial cells human tissue, 10 different 6
IF with confocal microscopy 50 patient samples
APC in nuclei of human colonic epithelial cells human tissue C-APC 28.9 7
Immunogold electron microscopy
Nuclear APC colocalizes with rRNA (nucleoli) 184A1 Ab-4 1
APC in nucleoli when ectopically expressed, and 3T3, HCT116 Ab-7 8
nuclear export inhibited by leptomycin B (LMB)