Fig. 1.

Hydrogen peroxide (H₂O₂) increases Nrf2 protein and activity in BEAS2B cells. (A) Cells were treated H₂O₂ (75 μM) for 0.5–24 h and nuclear Nrf2 was measured by Western blotting and normalized to the oxidant insensitive protein lamin C (n = 3). (B) The same nuclear extracts were used for measurement of Nrf2 DNA binding (n = 3). (C) Western blot analysis of BEAS2B cell nuclear extracts stimulated with H₂O₂ (1–100 μM) for 30 min (n = 3). (D) BEAS2B cells were stimulated with H₂O₂ (75 μM) for 8 h and HO-1 mRNA expression was normalized to GNB2L1 and analysed using qRT-PCR; NT: non-treatment. Values represent means ± SEM, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.005.