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. Author manuscript; available in PMC: 2012 Feb 11.
Published in final edited form as: Vaccine. 2011 Jan 1;29(8):1683–1689. doi: 10.1016/j.vaccine.2010.12.040

Figure 2.

Figure 2

Immunogold labeling of capsid display recombinant G2. Purified G2 and wild type Ad5 virus particles were reacted first with anti-NANP monoclonal antibody 2A10 and then with rabbit anti-mouse IgG secondary antibody conjugated to 2nM gold beads. Electron micrographs of negatively-stained samples show that recombinant (A) but not wild-type Ad5 (B) virions are reactive with the NANP antibody as indicated by decoration by the gold-labeled secondary antibody (arrows). The four virions shown are taken from different negatives. Brightness and contrast were adjusted individually for clarity and consistency of appearance.