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. Author manuscript; available in PMC: 2012 Feb 11.
Published in final edited form as: Vaccine. 2011 Jan 1;29(8):1683–1689. doi: 10.1016/j.vaccine.2010.12.040

Figure 6.

Figure 6

Capsid display recombinants induce neutralizing antibodies. Transgenic P. berghei sporozoites carrying the P. falciparum CSP NANP repeat were incubated with 1:6 dilutions of pre-immune serum, serum from Ad5- or G2-immunized mice, or CSP monoclonal antibody 2A10 (MAb). The treated sporozoites were added to liver cells in culture and parasite replication was measured 48h post-infection by qRT-PCR quantitation of P. berghei 18S rRNA in infected cells. Replication is expressed as the ratio between parasite rRNA and human actin mRNA for each RNA preparation. Ratios are the average of two biological replicates, each determined by three technical replicates. Error bars are the standard deviation of the mean of the two biological replicates. The monoclonal antibody and serum from G2-immunized mice reduced infectivity, while serum from Ad5-immunized mice did not. The right-most bar shows the 18S rRNA recovered from cells infected with killed (gamma-irradiated) sporozoites.