Figure 3.
Effect of alternative, non-alanine substitutions in the CDR3α of the HC/2G-1 TCR. Retroviral transduction, co-culture and IFN-γ assay was done as in Figure 1 and 2. WT is the native TCR and parental T-cell clone HC/2Tg-1 was also tested as a positive control. These studies identified AA positions 109 and 112 as loci with augmenting effects on function of this TCR.