Skip to main content
. Author manuscript; available in PMC: 2012 Mar 7.
Published in final edited form as: J Immunol Methods. 2011 Jan 19;366(1-2):43–51. doi: 10.1016/j.jim.2011.01.002

Figure 4.

Figure 4

Effects of combining enhancing AA substitutions at positions 109 and 112 in CDR3α. Dual AA substitutions were introduced into new retroviral vectors at positions 109 and 112. Transduction, co-culture and assay for IFN-γ production were same as previously described. The native TCR, GFP-transduced, and untransduced PBL were included as controls. The HC/2G-1 T-cell clone was also included in the assay. Repeated experiments were performed with multiple allogeneic PBL and gave similar results.

HHS Vulnerability Disclosure