Fig. 4.
Calcium signaling. Ad.AC6mut- and Ad.AC6-infected adult rat cardiac myocytes, unstimulated or stimulated with isoproterenol (1 μM, 24 h), were analyzed by real-time [Ca2+]cyt imaging. Representative Ca2+ transients in response to caffeine stimulation were recorded with Fura-2 fluorescence. Left, unstimulated cardiac myocytes expressing AC6 or AC6mut showed no change in [Ca2+]cyt in response to caffeine (10 mM). Number of cardiac myocytes assessed: control, 34; AC6, 24; AC6mut, 31. Right, isoproterenol-stimulated cardiac myocytes expressing AC6 or AC6mut showed increases in the peak amplitude of Ca2+ transient in response to caffeine stimulation. Number of cardiac myocytes assessed: control, 19; AC6, 24; AC6mut, 17 cells. (AC6 versus Null, ***, p < 0.001; AC6mut versus Null, **, p < 0.01).
