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Influence of temperature on [3H]Ry binding kinetics to R163C and WT preparations. Binding of 5 nM [3H]Ry to 100 μg/ml SR preparations was initiated at either 25 or 37°C and quenched at 5, 10, 15, 20, 25, and 30 min. The rate lines are plotted (A). The initial rates were obtained from linear curve fitting (B), and the calculated kobs values are plotted as bar graphs (A). Statistical analyses indicate significant difference among the compared groups (***, p < 0.001; n = 6–8).
