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. 2011 Mar;79(3):461–471. doi: 10.1124/mol.110.064816

Fig. 2.

Fig. 2.

MOR and DOR share pertussis toxin-sensitive G proteins. A, stimulation of [35S]GTPγS binding in membranes from SH-SY5Y cells after 60-min incubation with 1 μM DAMGO or SNC80 alone or in combination (DAMGO/SNC80). Incubation with DAMGO and SNC80 in combination (DAMGO/SNC80) did not significantly increase [35S]GTPγS binding more than DAMGO alone (p > 0.05 by one-way ANOVA with Bonferroni's post test) and stimulated significantly less [35S]GTPγS binding than the theoretical additive of the individual responses (R1 + R2) (**, p < 0.01 by one-way ANOVA with Bonferroni's post test). B, pretreatment of SH-SY5Y cells with PTX (100 ng/ml) for 24 h before membrane preparation blocked the stimulation of [35S]GTPγS binding by 1 μM DAMGO, SNC80, or the combination (DAMGO/SNC80) (***, p < 0.001 by two-way ANOVA with Bonferroni's post test). Pertussis toxin treatment did not alter spontaneous [35S]GTPγS binding in the absence of agonist (p > 0.05 by two-way ANOVA with Bonferroni's post test). Data are presented as mean ± S.E.M. (n = 3, in triplicate); n.s., not significant.