Receptor density in differentiated SH-SY5Y cells
Receptor numbers in cell membranes were determined using selective agonist radioligands at a maximal concentration as described under Materials and Methods. 5-HT1A was not tested. Binding of maximal concentrations of antagonists for MOR ([3H]diprenorphine in the presence of the δ-selective ICI 174,864), DOR ([3H]naltrindole), and α2AR ([3H]yohimbine) was 103 ± 8.0, 98 ± 11, and 79 ± 6% of the agonist binding, respectively. Results are presented as mean ± S.E.M. (n = 3–6).