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. 2001 Mar 13;98(6):3104–3108. doi: 10.1073/pnas.051635998

Figure 2.

Figure 2

A representation of the cDNA of human FAS DI and DII-DIII plasmids used in identifying the importance of the ID region in the FAS dimer formation. The top bars represent, respectively, human FAS monomer and dimer. The cDNA nucleotide sequences coding for various constituent activities (speckled bars) and the ID (filled bars) are indicated. The N- (ID-N) and C-terminal (ID-C) halves of the ID are represented as gray and black bars, respectively. The human FAS cDNA nucleotide numbers on the top bar indicate the location of the restriction sites for BamHI (B), HindIII (H), and NotI (N), and the boundaries of the coding sequences present in the recombinant expression plasmids. The cartoon describing the FAS dimer with two active centers is drawn by using the descriptors shown for human FAS cDNA shown in the top line. The construction of the plasmids used for the expression of human FAS recombinant protein halves containing ID halves is described as before (23, 24), except that their names have been changed to include the ID sequences present in them. The constructs lacking ID regions, TRX-hFAS-DI and TRX-hFAS-DII-III, are described in Materials and Methods. TRX, thioredoxin (as N-terminal fusion present in the recombinant protein).