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. 2001 Mar 13;98(6):3104–3108. doi: 10.1073/pnas.051635998

Figure 3.

Figure 3

(A) A schematic representation of how human FAS ID fusions of GAL4-DB (filled rectangle) and GAL4-ACT (filled oval) might interact to reconstitute the GAL4 transcription factor to activate the β-galactosidase gene. The head–tail interaction of ID regions will bring together DB and ACT to function as a “native” GAL4 transcription factor that binds to the upstream activation sequence (UAS) and activates transcription of the β-galactosidase gene. (B) Description of plasmids used in the yeast two-hybrid system for determining the regions in the FAS monomer that are involved in the dimer formation. The yeast two-hybrid system vectors pAS2 and pACT2 contain the N-terminal DB (GAL4-DB) and the C-terminal ACT (GAL4-ACT), respectively. The construction of the yeast two-hybrid system plasmids containing the human FAS sequences fused in-frame with the C terminus of GAL4-DB and GAL4-ACT coding sequences is described in Materials and Methods. The depiction of various bars and the significance of nucleotide numbers, etc., are as described in Fig. 2.