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. Author manuscript; available in PMC: 2012 Mar 3.
Published in final edited form as: Vaccine. 2011 Jan 6;29(11):2131–2139. doi: 10.1016/j.vaccine.2010.12.087

Figure 6.

Figure 6

Mucosal cellular IFN-γ response to OprF following mucosal immunization. AdC7OprF, AdOprF, Ad5Null or AdC7Null were administered intratracheally at a dose of 1010 pu/mouse. Eight wk following administration, CD3+ T cells were isolated from lungs and incubated in vitro with splenic or lung DC alone or pulsed with recombinant OprF and OprF-specific IFN-γ responses were determined in an ELISPOT assay. A. INF-γ in lung CD3+ T cells stimulated with pulsed syngenic pulmonary DC. B. INF-γ in lung CD3+ T cells stimulated with pulsed syngenic splenic DC. The data represent the mean of pooled cells from five mice per group (n =5). Values are presented after subtraction of background IFN-γ secretion by corresponding CD3+ T cells induced by DC alone. *, ** and *** denote significance of p<0.05, p<0.01 and p<0.001, respectively.