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. Author manuscript; available in PMC: 2011 Dec 22.
Published in final edited form as: Mol Cell. 2010 Dec 22;40(6):1001–1015. doi: 10.1016/j.molcel.2010.11.032

Figure 6. The Crossover Function of Exo1 Involves Interaction With Mlh1.

Figure 6

(A) Domain structure of EXO1 and the four alleles analyzed.

(B) Physical analysis of crossing-over in exo1 mutant strains.

(C) Quantitative analysis of crossing-over in exo1 mutant strains. For each strain, at least three independent time-course experiments were analyzed (bars show the mean value ± S.E. for the 13 hr time-points, when crossover levels plateau).

(D) Analysis of DSB resection in wild-type and exo1-FF477AA cells.

(E) Space filling model of Mlh1 showing the position of the S2 interaction site and E682 residue. The S1 site mediates heterodimerization with the other MutL homologs, Pms1, Mlh2 and Mlh3.

(F) Physical analysis of crossing-over in wild-type, mlh1-E682A and mlh1Δ cells.

(G) Quantitation of crossover levels in wild-type, mlh1-E682A and mlh1Δ cells. Graphs show the averages of at least three independent time courses (means ± S.E. for the 13 hr time-points).

(H) Physical assay to detect unrepaired heteroduplex DNA.

(I) Analysis of heteroduplex repair in wild-type, mlh1-E682A and mlh1Δ cells.

(J) Quantitation of heteroduplex levels in wild-type, mlh1-E682A and mlh1Δ cells. Graphs show the averages of at least three independent time courses (means ± S.E. for the 13 hr time-points).