Skip to main content
. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2011 Feb;70(2):110–115. doi: 10.1097/NEN.0b013e31820565f9

Figure 1.

Figure 1

World Health Organization grade II diffuse astrocytoma immunoreactive for p53 and mutant isocitrate dehydrogenase 1 (IDH1) and with copy number gain of chromosome 7. (A) p53 immunohistochemistry (IHC) shows strong, diffuse, nuclear positivity. (B) Mutant-specific IDH1 (R32H) IHC shows strong granular cytoplasmic and sometimes nuclear immunoreactivity in infiltrating tumor cells in the background of normal non-neoplastic brain parenchyma. (C) Fluorescence in situ hybridization analysis for chromosome 7 copy number detects copy number gain for two probes (Spectrum Red EGFR probe, Spectrum Aqua centromere 7). Similar results were obtained with the C-MET probe (data not shown). The majority of cases showed gain of all 3 markers, likely representing gain of the entire chromosome.