FIG. 8.

Effect of MXC, MOH, and HPTE on gene expression levels of Cyp1a2 (MXC metabolizing enzyme) in antral follicles of control and ESR1 OE mice. Antral follicles were cultured with MXC (1–100 μg/ml), MOH (0.1–10 μg/ml), HPTE (0.1–10 μg/ml), or the vehicle DMSO for 96 h and then subjected to q-PCR with Cyp1a2-specific primers. The relative gene expression quantities of Cyp1a2 were normalized to β-Actn. (A) MXC-treated follicles; (B) MOH-treated follicles; (C) HPTE-treated follicles. Each bar represents means ± SEM. Bars with different letters are significantly different from each other (n = 13–15 follicles per treatment from three separate experiments; p ≤ 0.05).