FIG. 6.

Accumulation of [¹⁴C]CDCA (A, B, and C) or [³H]TCA (D, E, and F) in suspended rat hepatocytes in the presence of 1μM [¹⁴C]CDCA or 1μM [³H]TCA and vehicle (•,○ 0.3% DMSO; CTL), 10μM TRO (▪,□), or 50μM MK571 (▴,▵). Lines represent the linear regression of the data using GraphPad Prism 5.03. Total accumulation (Na⁺-dependent and independent) was measured in Na⁺-containing buffer (A and D). Na⁺-independent uptake was measured in choline-containing buffer (B and E). Na⁺-dependent uptake was calculated by subtracting Na⁺-independent accumulation from total accumulation (C and F; open symbols and dashed lines). Data represent the mean ± SE of triplicate determinations in n = 3 experiments; †p < 0.05 versus CTL for TRO, and ‡p < 0.05 versus CTL for MK571.