TABLE 1.
Transitions Monitored at Unit Resolution for LC-MS/MS Analysis of Parent CDCA and Taurine- and Glycine-Conjugated CDCA Metabolites in Cell Lysates from WT Rat SCH Following a 10-min Incubation with 1μM CDCA
| Analyte | Molecular weight | Salt | Retention time (min) | Precursor m/z | Product m/z | Calibration curve range |
| TCA | 515.7 | None | 5.7 | 514 | 124 | n/a |
| d4-TCA | 519.7 | None | 5.7 | 514 | 124 | Internal standard |
| d8-TCA | 545.73 | Na+ | 5.7 | 522 | 128 | 0.5–200 pmol per well |
| GCA | 465.62 | None | 5.8 | 464 | 74 | n/a |
| d4-GCA | 469.65 | None | 5.8 | 468 | 74 | 0.5–100 pmol per well |
| TCDCA | 499.7 | None | 8.1 | 498 | 80 | n/a |
| d4-TCDCA | 503.73 | None | 8.1 | 502 | 80 | 0.5–100 pmol per well |
| GCDCA | 449.62 | None | 8.1 | 448 | 74 | n/a |
| d4-GCDCA | 453.65 | None | 8.1 | 452 | 74 | 0.5–100 pmol per well |
| CDCA | 392.57 | None | 10.2 | 5–1000 pmol per well |