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. 2010 Nov;51(11):5499–5507. doi: 10.1167/iovs.10-5543

Figure 3.

Figure 3.

Deletion analysis of 5′ flanking region of the human CTRP5 gene promoter. Luciferase assay was performed on the CTRP5 promoter deletion constructs where −386 bp to −29 bp (358 bp), −675 bp to −29 bp (647 bp), −1041 bp to −29 bp (1013 bp), −1322 bp to −29 bp (1350 bp), and −3569 bp to −29 bp (3.5 kb) nucleotides upstream of the ATG start sites were cloned in the pGl3-Enhancer vector (pGl3E) and only −3569 bp to −29 bp (3.5 kb) was cloned in pGl3Basic (pGl3B) vectors. The promoterless plasmids pGl3Basic and pGl3 Enhancer were used as negative controls, and the plasmid pGl3Control containing the SV40 promoter was used as positive control for the experiment. The pRL-SV40 vector containing the SV40 early promoter upstream region of Renilla luciferase was used as an internal control for the normalization of transfection efficiency. Data represent mean ± SD from at least three independent experiments.