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. 2010 Nov;51(11):5825–5832. doi: 10.1167/iovs.10-5576

Figure 5.

Figure 5.

Parstatin reduces VEGF-induced retinal leukostasis. Intravitreal injections of vehicle (control), VEGF (10 μΜ), parstatin (parst, 10 μg), or the combination of VEGF with parstatin were administered to assess their effect on retinal leukostasis. After 6 hours, mice were perfused with FITC-conjugated concanavalin A. Retinal flatmounts were prepared, and the total number of leukocytes adhering to the retinal vessels (arrows) was counted at 200× magnification. Few leukocytes adhered to the retinal vessels after injections of vehicle or parstatin (A, 200×; inset, 400×), whereas pronounced leukostasis was observed after injections of VEGF (B, 200×; inset, 400×). The combination of parstatin with VEGF resulted in a significant reduction in VEGF-induced leukostasis (C, 200×). Scale bar, 200 μm. (D) Total numbers of adherent leukocytes were measured in each retina. Results are expressed as mean number of leukocytes per section ± SE for each group calculated from the indicated number (n) of eyes. Statistical analysis was performed compared with the control group. *P < 0.05.