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. 2010 Nov;51(11):5991–6000. doi: 10.1167/iovs.10-5395

Figure 4.

Figure 4.

Comparison of proliferation of Tag-induced and noninduced ImM10 cells in culture. (A) Passages 4 to 7 and (B) passages 16 to 18 cultured under immortalizing (open squares; 33°C, GM+IFNγ) or non-immortalizing (closed squares; 39°C, GM without IFNγ) conditions showing mean fold-change in live cells (calcein AM positive, ethidium bromide homodimer negative). Error bars show SEM. Early-passage cells were not counted on days 1 to 6 because visual observations and pilot studies showed minimal changes in cell number before 7 days in culture. (C) Quantitative RT-PCR comparing mRNA expression between cells cultured under immortalizing conditions (33°C) and nonimmortalizing conditions (39°C). Image shows ethidium bromide–stained agarose gels of amplicons. Table shows fold-change and P-values.