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. 2010 Nov;51(11):6038–6050. doi: 10.1167/iovs.10-5443

Figure 2.

Figure 2.

Western blot analysis of single samples from normal (16 and 7 weeks) and XLPRA2 mutant (16 and 7 weeks) retinas with four antibodies (NDUFS4, SAG, FAP, and OPN1SW) in addition to actin (ACTB), used as a loading control. Dashed line indicates that the gel was cut and ACTB placed below the protein of interest. The relevant molecular size markers are indicated. (A) NDUFS4 levels were lower in the mutant retina at both ages. (B) Lower protein levels of SAG were found in the mutant retina at 16 weeks compared with either the normal age-matched control or the 7-week mutant. (C) GFAP was upregulated in both the 7- and 16-week mutant retinas. (D) The mutant retina at 16 weeks showed a downregulation of OPN1SW. The specific ∼39 kDa band is absent, even though the quantity of the ACTB loading control was high.