Fig. 1.

Expression of Oatp1a4 protein in rat brain microvessels. Top, Western blot analysis of brain microvessels isolated from rats administered 0.9% saline or 3% λ-carrageenan by subcutaneous injection into the plantar surface of the right hind paw. Crude membrane preparations (10 μg) from rat brain microvessels were resolved on a 10% lithium dodecyl sulfate-polyacrylamide gel, transferred to a PVDF membrane, and analyzed for expression of Oatp1a4 (M50 antibody; 1:500 dilution). Oatp1a4 in brain microvessels and liver lysate from naive rats is shown as a positive control. Bottom, Oatp1a4 expression in λ-carrageenan-treated animals and saline controls was determined by densitometric analysis. Results are expressed as mean ± S.D. of three separate experiments. Asterisks represent data points that are significantly different from control.