Fig. 8.
The concentration-response curves of KRS-5Me-4-OCF3-evoked inhibition and GABA in the presence of KRS-5Me-4-OCF3. A, the KRS-5Me-4-OCF3-evoked change in spiking rate was normalized to the control condition, and then averaged. Each point was the mean value ± S.E.M. of four to seven cells. The line is fit for the data to the Hill equation: y = Y0 + Axn/(Kan + xn), where y is the inhibition of spiking rate, x is concentration of drugs, Y0 is minimal inhibition, A is maximal inhibition, Kd is the apparent dissociation constant for agents, and n is the Hill coefficient. Kd and n were estimated using a Marquadt nonlinear least-squares routine. B, shift of the concentration-response curves of GABA in the presence of KRS-5Me-4-OCF3 at different concentrations (0, 5, and 20 μM). The lines are fits for the data to the Hill equation.