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. 2008 Feb 18;8:10. doi: 10.1673/031.008.1001

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Figure 2. Images of a 20-day old Manduca sexta pupa. The orientation of the PDW and their corresponding FISP images is identical, so the orientation is only displayed on the former. For the PDW images: A = Anterior, D = Dorsal, L = Left, P = Posterior, R = Right, V = Ventral. The white arrows connecting the FISP images indicate the time that elapsed between the displayed images. A) Axial PDW image. The red arrow is pointing towards the location of the dorsal vessel. The two large dark areas on the right and left side of the body are the air sacs. The slice location of A—C was 8.0 mm below the end of the proboscis. B—C) Individual frames from Movie 1; the frames were acquired 632 ms apart. The red arrows in both images point towards the dorsal vessel and ventral diaphragm, respectively. D) Sagittal PDW image. The dark area ventral to the middle arrow is part of an air sac. The slice location of D had an anterior edge that began at the end of the proboscis. The ventral side of the slice is 3 mm to its right, while the dorsal side of the slice intersected the dorsal vessel. The red arrows are pointing towards the location of the dorsal vessel. E—F) Individual frames from Movie 2 at the same location and orientation as (D); the frames were acquired 1264ms apart. The red arrow in both images point towards where hemolymph is observed flowing through the dorsal vessel in (F). See also Movie 1 and Movie 2.

Movie 1. Each frame is separated by 316 ms. Movie 1 corresponds to Figure 2A-C. See Figure éfor orientation and structural information. Hemolymph flow through the dorsal vessel and ventral diaphragm is seen throughout the movie. This video clip can be accessed at http://digital.library.wisc.edu/1793/23227

Movie 2. Each frame is separated by 316 ms. Movie 2 corresponds to Figure 2D—F. See Figure 1 for orientation and structural information and the text for a detailed description. Several pulses of hemolymph flowing through the dorsal vessel are observed on the right side of the movie, which is the dorsal side of the pupa. This video clip can be accessed at http://digital.library.wisc.edu/1793/23226