Figure 4.

Images of a 21-day old Manduca sexta pupa. The orientation of the PDW and their corresponding FISP images is identical, so the orientation is only displayed on the former. For the PDW images: A = Anterior, D = Dorsal, L = Left, P = Posterior, R = Right, V = Ventral. The white arrows connecting the FISP images indicate the time that elapsed between the displayed images. A) Coronal PDW image. The red arrows identify part the gut. The slice location of B—D was 6.3 mm behind the end of the proboscis and the anterior boundary is 2 mm above it. B—D) Individual frames from Movie 5 at the same location and orientation as (A); the frames were acquired 1264 ms apart. The red arrows in the images identify gut and follow a peristaltic wave moving in the posterior direction. See also Movie 5.

Movie 5. Each frame is separated by 316 ms. Movie 5 corresponds to Figure 4A—D. See Figure 4A for orientation and structural information and the text for a detailed description. Peristalsis of the gut is visible in the opening frames of the movie. This video clip can be accessed at http://digital.library.wisc.edu/1793/23223