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. 2008 Apr 1;8:27. doi: 10.1673/031.008.2701

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression profiles of polygalacturonase genes of Lygus lineolaris. (A-C) Relative semiquantitative RT-PCR values, as determined by gel density analysis and normalized to cytoplasmic actin, 16S ribosomal, and S2 ribosomal standards. Vertical bars connect the two expression levels in separate experimental replicates, horizontal crossbars indicate the average. EggD3: 3 day-old eggs, eggD8: 8 day-old eggs, nymphI2: second instar (small) nymphs, nymphMI5: fifth instar male nymph, nymphFI5: fifth instar female nymph, adultM: male adult, adultF: female adult. (A) Llpg1, (B) Llpg2, (C) Llpg3. (D) Semiquantitative RT-PCR gels of additional adult specimens. F: female, M: male, O: no template, PG1: Llpg1, PG2: Llpg2, PGs: Llpg3, S2: S2 ribosomal standard.

continued — Expression profiles of polygalacturonase genes of Lygus lineolaris. (A-C) Relative semiquantitative RT-PCR values, as determined by gel density analysis and normalized to cytoplasmic actin, 16S ribosomal, and S2 ribosomal standards. Vertical bars connect the two expression levels in separate experimental replicates, horizontal crossbars indicate the average. EggD3: 3 day-old eggs, eggD8: 8 day-old eggs, nymphI2: second instar (small) nymphs, nymphMI5: fifth instar male nymph, nymphFI5: fifth instar female nymph, adultM: male adult, adultF: female adult. (A) Llpg1, (B) Llpg2, (C) Llpg3. (D) Semiquantitative RT-PCR gels of additional adult specimens. F: female, M: male, O: no template, PG1: Llpg1, PG2: Llpg2, PGs: Llpg3, S2: S2 ribosomal standard.