Table 1.

Statistical analysis of U values.

	0%-100% (a1=0, a2=100)				5%-95% (a1=5, a2=95)				10%-90% (a1=10, a2=90)
	Avrg	SEM	p-value	Grubb test	Avrg	SEM	p-value	Grubb test	Avrg	SEM	p-value*	Grubb test
ALA	1.0946	0.0435	< 0.0001	0.982	1.198	0.042	< 0.0001	0.954	1.244	0.050	< 0.0001	0.970
ARG	1.8177	0.1620	< 0.0001	0.193	2.170	0.121	< 0.0001	0.103	2.095	0.096	< 0.0001	0.135
ASN	1.9614	0.1184	< 0.0001	0.036	2.320	0.126	< 0.0001	0.029	2.467	0.137	< 0.0001	0.230
ASP	1.5031	0.0728	< 0.0001	0.536	1.724	0.088	< 0.0001	0.495	1.619	0.050	< 0.0001	0.602
CYS	4.5809	0.3846	-	2.822	5.376	0.281	-	2.712	4.835	0.279	-	2.555
GLN	2.0750	0.1164	< 0.0001	0.088	2.499	0.189	< 0.0001	0.186	2.776	0.205	< 0.0001	0.534
GLU	1.2493	0.0583	< 0.0001	0.813	1.246	0.078	< 0.0001	0.914	1.194	0.060	< 0.0001	1.020
GLY	1.9518	0.2277	< 0.0001	0.047	2.912	0.316	< 0.0001	0.549	2.641	0.199	< 0.0001	0.401
HIS	3.0330	0.2264	0.0022	1.133	3.885	0.244	0.0014	1.403	3.742	0.190	0.0038	1.482
ILE	1.1824	0.0366	< 0.0001	0.886	1.100	0.026	< 0.0001	1.043	1.124	0.033	< 0.0001	1.088
LEU	1.1723	0.0980	< 0.0001	0.897	1.161	0.081	< 0.0001	0.989	1.136	0.070	< 0.0001	1.076
LYS	1.2946	0.0682	< 0.0001	0.763	1.316	0.062	< 0.0001	0.853	1.393	0.061	< 0.0001	0.824
MET	2.7132	0.2046	0.0003	0.784	3.138	0.205	< 0.0001	0.747	3.151	0.212	< 0.0001	0.902
PHE	1.9166	0.0821	< 0.0001	0.085	2.096	0.089	< 0.0001	0.167	1.998	0.074	< 0.0001	0.230
PRO	2.2688	0.3933	< 0.0001	0.299	2.592	0.196	< 0.0001	0.268	2.371	0.135	< 0.0001	0.136
SER	1.5879	0.0914	< 0.0001	0.444	1.770	0.115	< 0.0001	0.454	1.958	0.124	< 0.0001	0.269
THR	1.5873	0.0675	< 0.0001	0.444	1.863	0.075	< 0.0001	0.372	1.965	0.074	< 0.0001	0.263
TRP	3.7128	0.1699	0.0499	1.875	4.057	0.184	0.0019	1.554	3.717	0.273	0.009	1.457
TYR	2.1025	0.1206	< 0.0001	0.118	2.240	0.110	< 0.0001	0.041	2.125	0.096	< 0.0001	0.105
VAL	1.0832	0.0251	< 0.0001	0.994	1.077	0.024	< 0.0001	1.062	1.099	0.029	< 0.0001	1.112

Statistical analysis of U values. The analysis used Eq. 1 and considered different intervals for the definition of degeneration (a₁ parameter in Eq. 1) and conservation (a₂ parameter in Eq. 1). Avrg, indicates average value found for ModBase homology models separated by organism (13 organisms). SEM, standard error of means (p-value calculated with n=13); in the Grubb test column, the results of the Grubb's test for outlier, with critical Z-score (for N=20) of 2.71. Outliers with p-value <0.05 are shown in bold.

^*The p-value refers to pairwise comparison of calculated values (e.g., “ModBase Avrg” values), between Cys and each one of the other amino acids.