Skip to main content
. Author manuscript; available in PMC: 2011 Mar 21.
Published in final edited form as: J Inorg Biochem. 2007 Jul 16;101(11-12):1692–1698. doi: 10.1016/j.jinorgbio.2007.07.007

Figure 2.

Figure 2

The in vivo partitioning and uptake study of apo-ME and Fe-ME with the Marinobacter sp. strain DS40M6. DS40M6 was grown to late log phase (OD600 0.65) in 2 L of ASW medium. The cells were centrifuged and resuspended in fresh ASW medium (OD600 0.68) and allowed to equilibrate for a total of 30 min. Descriptors of cells and conditions are as follows: medium control, ASW medium with 20 μM siderophore mixture (apo-ME and Fe-ME); active cells, 20 μM siderophore mixture added after 30 minute equilibration of cells; dead cells, 1% glutaraldehyde added 30 min prior to addition of 20 μM siderophore mixture; and metabolically inhibited cells, 5 mM KCN added 10 min prior to addition of 20 μM siderophore mixture. Siderophore-cell mixtures were incubated for 1.5 h. The cells were ultracentrifuged and the quantities of apo-ME and Fe-ME remaining in the supernatant were determined by HPLC.