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. 2011 Mar 21;6(3):e17761. doi: 10.1371/journal.pone.0017761

Figure 5. N-Mis17 overproduction causes sensitivity to TSA.

Figure 5

A. Wild type, mis6-302, mis15-68, and mis17-362 were spotted on a plate in the absence or presence of 25 µg/ml TSA at 26–36°C. See text. B. Wild-type cells that overproduced N-Mis17 by REP41 or REP81 were plated in the absence or presence of TSA (0–25 µg/ml) at 30°C. C. The N-Mis17 fragment contains eight K residues that were substituted to R. The resulting N-Mis17-8R gene was expressed under the REP41 promoter in the wild-type strain. D. The level of overproduced Mis17-N 8R was assayed compared with N-Mis17. PSTAIRE was the level of Cdc2 protein kinase assayed by the antibody PSTAIRE.