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A. The negative dominance effect of N-Mis17 overexpression was greatly diminished in six protein kinase deletion mutants, Δssp2, Δppk9, Δppk15, Δppk30, Δlsk1, and Δwis4. Promoter is off or on in the presence or absence of thiamine, respectively. B. A cartoon for the implication of Mis17 in the Mis6-Mal2-Sim4 complex and negative dominance effect of the N-Mis17 fragment. See text.
