Figure 5. Antibiotic gavage treatment alters gene expression profile of the colonic epithelium.

Gene expression profile of colonic epithelial cells isolated from mice subjected to 17 days of treatment with vancomycin, neomycin, metronidazol, and amphotericin-B every 12 hours by gavage with ampicillin ad libitum in drinking water (Treated) compared to mice gavaged with water only (Untreated). All Treated mice exhibited successful depletion of cultivable intestinal microbiota (<1 cfu/mg feces). (A) Heat-map analysis of significantly (p<0.05) differentially expressed genes with >two-fold change organized in dendrogram according to Eukledian relation of the differentially expressed genes. Colors indicate absolute expression of one gene estimated by mean values of the multiple probes detecting each gene on the chip. Each row represent one gene, each column represent one mouse. (B) Gene ontology map folder enrichment analysis of differentially expressed genes in MetaCore (GeneGo, St Joseph, MI).