Skip to main content
. 2011 Mar 21;6(3):e17997. doi: 10.1371/journal.pone.0017997

Figure 1. BRM controls expression of CO, FT and SOC1 genes.

Figure 1

A) Analysis of CO and FT expression in wild-type (Col), brm-1 and brm-2 mutant plants by RT-PCR. Total RNA was isolated from seedlings collected 10 h after dawn at 12 days of growth under LD conditions. GAPC transcript levels were also determined as a control for the amount of input cDNA. B) GUS expression patterns of gCO::GUS and pFT::GUS in wild-type and BRM-silenced plants (brm29.1) in whole-mount staining of 6-day-old and 12-day-old seedlings under LD conditions. C) Flowering time of plants grown under LD photoperiod. Data are means and standard deviation of at least 20 plants. Differences between the indicated pairs of data are significant with p<0.05 (*) or p<0.01 (**). D) Analysis of SOC1 expression in wild type (Col), ft-10, ft-10 brm-2 and brm-2 plants by qRT-PCR. Total RNA was isolated from seedlings collected 10 h after dawn at 14 and 15 days of growth under LD conditions. E) Analysis of FT expression in co-10 and co-10 brm-2 mutant plants by qRT-PCR. Total RNA was isolated as in D.