Table 3.
Summary of parameter values used for model simulations.*
| Parameter | Value | Units |
|---|---|---|
| D | 0.3 | h-1 |
| Ginmax | 0.036 | g plasmid DNA/L biomass |
| Ks | 0.005 | g/L |
| kd | 0.46 | min-1 |
| ke | 0 | min-1 |
| kp0 | 2400/(233 μ-2 + 78) | min-1** |
| kq0 | 3600α/(82.5 μ-1 + 145) | min-1** |
| α = 1 for μ > ln2 | ||
| α = μ/ln2 for μ < ln2*** | ||
| m | 2.399**** | - |
| Np | 41 (pP1-(SD)-hIFNγ) | - |
| 24 (pP1-(4SD)-hIFNγ) | ||
| 30 (pP1-(ΔSD)-hIFNγ) | ||
| n | 1 | - |
| pinmax | 150 | g protein/L dry biomass |
| sF | 1.96 | g/L |
| μmax | 0.66 | h-1 |
| ρB | 1000 | g dry biomass/L dry biomass |
*In the absence of translation (as in pP1-(ΔSD)-hIFNγ) the gene expression parameters are ignored.
**Calculating kp0 and kq0 the specific growth rate μ is expressed in h-1.
***By α = μ/ln2 (for μ < ln2) and Eq. (9), the substitution of kp0, kq0 and kd in Eq. 11 results in Eq. 15.
****The m value was determined by nonlinear fitting for pP1-(ΔSD)-hIFNγ and set in the calculations for both pP1-(SD)-hIFNγ and pP1-(4SD)-hIFNγ (see below).