Table 5.
Enzymes involved in TAG biosynthesis and catabolism identified by annotation of the D. tertiolecta transcriptome
| Enzyme | Symbol | EC Number | Number of transcripts | 1%Sequence alignment with corresponding enzymes in model organisms (Accession #) | |
|---|---|---|---|---|---|
| C. reinhardtii | V. carteri | ||||
| TAG biosynthesis | |||||
| Glycerol kinase | GK | 2.7.1.30 | 16 | 42 (A8IYH1) | NM |
| Glycerol-3-phosphate O-acyltransferase | GPAT | 2.3.1.15 | 0 | 2NM | NM |
| 1-Acyl-sn-glycerol-3-phosphate O-acyltransferase | AGPAT | 2.3.1.51 | 2 | NM | NM |
| Phosphatidate phosphatase | PP | 3.1.3.4 | 1 | NM | NM |
| Diacylglycerol O-acyltransferase | DGAT | 2.3.1.20 | 2 | NM | NM |
| Phospholipid:diacyglycerol acyltransferase | PDAT | 2.3.1.158 | 1 | NM | NM |
| TAG catabolism | NM | NM | |||
| Triacylglycerol lipase | TAGL | 3.1.1.3 | 2 | NM | NM |
1In cases where multiple transcripts have been aligned with the associated enzymes in the model organisms, average similarity is reported.
2NM denotes that the annotated transcripts did not match the sequence of corresponding enzyme in model organisms.