Figure 2.

Graphical representation of protein spots down-regulated (A) and up-regulated (B) in sera from CSFV-infected pigs (p < 0.01). Protein spot detection and quantification were performed by the DIA (Differential In-gel Analysis) module of the DeCyder 6.5 software. Each circle represents the abundance of the referred spot in an individual gel, which was expressed as a volume ratio to its corresponding internal standard (spot labeled with Cy2 in the same gel). For each group (control and CSFV-infected pigs), the arithmetic mean of the standardized spot abundances is indicated by the symbol +. To match protein spots across multiple gels and perform statistical analysis (paired Student's t test), the BVA (Biological Variation Analysis) module was used.