Table 1.
Proteins with at least 1.5-fold quantitative alteration in expression in serum from CSFV-infected pigs measured by 2-D DIGE and MALDI-ToF MS analysis
| Spotno.a | Identified proteins | GI no.b | Ratio (I/U)c | MMd (kDa)/pI | M/Se | Seq Cov (%)f | Scoreh | t-tes P value |
|---|---|---|---|---|---|---|---|---|
| 632 | similar to RNA binding motif protein 15B | gi| 126336349 | -2.1 | 99/9.9 | 7/12 | 9 | 71 | 0.004 |
| 636 | serotransferrin | gi| 136192 | 1.5 | 79/6.9 | 7/14 | 15 | 81 | 0.003 |
| 808 | MGF 505-3R | gi| 162849409 | 1.6 | 12/9.2 | 5/16 | 52 | 75 | 0.005 |
| 827 | vitamin D-binding protein | gi| 51863317 | -2.1 | 25/5.0 | 9/19 | 33 | 126 | 0.001 |
| 1036 | retinol-binding protein 4 | gi| 47522930 | 1.7 | 23/5.4 | 6/14 | 31 | 82 | 0.004 |
| 1098 | similar to thrombin inhibitor isoform 2 | gi| 119915930 | -1.5 | 42/5.6 | 5/11 | 28 | 78 | 0.002 |
a) Labels of protein spots in Figure 1.
b) GI no. is MASCOT results of MALDI-ToF MS from the NCBInr database.
c) Relative protein expression in infected/uninfected samples.
d) MM, Molecular mass.
e) Number of mass values matched/Number of mass values searched.
f) Sequence coverage (%), the percentage of sequence covered versus the size of the matched protein.
h) Probability based Mowse score.