Table 1.

Time course of amyloid formation for wild-type IAPP and the F_C≡N variants as determined from the thioflavin-T-detected kinetic experiments

	t50 (s)	tlag (s)	${\mid \frac{\text{d}F(t)}{\text{d}t}\mid }_{t=t_{50}}({\text{s}}^{-1})$	vmax (nM/s)	$t_{50}^{\text{s}}/t_{50}$
15FC≡N	899±10	841±16	4.48±0.71×10−3	158±3	0.087
23FC≡N	967±41	875±40	3.12±0.08×10−3	155±15	0.094
37FC≡N	1013±100	935±75	4.15±0.11×10−3	142±8	0.096
WT	960±60	870±66	4.86±0.21×10−3	168±13	—

Experiments were performed at 25 °C, pH 7.4, and 2% HFIP with constant stirring. The quoted uncertainties represent the standard deviation calculated from three independent runs.