Table 2.
Ratio of final to initial fluorescence | t50 (s) | tlag (s) | vmax (nM/s) | |||
---|---|---|---|---|---|---|
15FC≡N | 0.44±0.01 | 876±10 | 811±20 | 6.73±1.0×10−3 | 258±17 | |
23FC≡N | 0.30±0.01 | 957±41 | 842±44 | 3.82±0.41×10−3 | 168±12 | |
37FC≡N | 0.21±0.02 | 997±100 | 909±85 | 3.5±0.19×10−3 | 151±15 |
The quoted uncertainties represent the standard deviation calculated from three independent runs.