Table 2.
Pathological evalutation of H&E stained plantaris muscles
| Control | 1 Day | 3 Days | 5 Days | 7 Days | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Young | Old | Young | Old | Young | Old | Young | Old | Young | Old | |
| Muscle Pathology | ||||||||||
| Degeneration | ||||||||||
| area of slide | 0.0 | 0.1±0.0 | 3.3±0.3 | 4.0±0.0 | 4.0±0.0 | 2.7±0.8 | 2.7±0.6 | 2.5±0.7 | 1.4±0.5 | 1.2±0.5 |
| severity | min-mod | min-mod | min-mod | mld-sev | min-mod | min-mod | min | min-mld | ||
| Necrosis | 0.0 | 0.0 | 1.0±0.3 | 1.3±0.6 | 1.7±0.7 | 1.5±0.7 | 0.8±0.5 | 0.7±0.4 | 0.1±0.1 | 0.0 |
| Edema | 0.0 | 0.0 | 1.5±0.4 | 2.3±0.3 | 1.7±0.4 | 1.2±0.4 | 0.7±0.5 | 0.5±0.3 | 0.1±0.1 | 0.2±0.2 |
| Fibrosis | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.6±0.4 | 1.5±0.5 | 1.7±0.4 | 2.0±0.3 |
| Hemorhage | 0.0 | 0.0 | 0.3±0.2 | 1.7±0.5 | 2.5±0.3 | 0.7±0.3 | 1.3±0.5 | 2.3±0.2 | 0.0 | 0.2±0.2 |
| Regeneration | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 1.2±0.6 | 3.2±0.7 | 2.9±0.6 | 4.0±0.0 |
| Inflammation | ||||||||||
| Neutrophils | 0.0 | 0.0 | 2.0±0.3 | 2.7±0.2 | 2.2±0.5 | 1.7±0.6 | 1.0±0.4 | 1.8±0.3 | 0.3±0.2 | 0.2±0.2 |
| Macrophages | 0.0 | 0.0 | 0.3±0.2 | 0.5±0.2 | 1.0±0.5 | 0.7±0.4 | 1.7±0.6 | 2.3±0.3 | 1.0±0.3 | 1.6±0.4 |
| Lymphocytes/plasma cells | 0.0 | 0.0 | 0.3±0.2 | 0.2±0.2 | 0.5±0.3 | 0.0 | 0.8±0.4 | 1.7±0.2 | 1.7±0.2 | 1.6±0.4 |
For measures of degeneration and necrosis: 0 = normal muscle, 1 = 0–5%, 2 = 5–20%, 3 = 20–40%, and 4 = >40% of slide area; severity of degeneration is denoted as min (minimal), mld (mild), mod (moderate), or sev (severe). For edema, hemorhage, fibrosis, neutrophils, macrophages, and lymphocytes/plasma cells: 0 = normal, 1= minimal, 2 = mild, 3 = moderate, and 4 = severe. Values indicated are means ± SE.